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Flow chamber system FCS
The bottom of the flow channel is formed by the corpus, the side wall – by the gasket, and its top – by the cover slip. The cover slip may be pre-coated with a cell monolayer in a multi-well plate, and will be fixated on the flow chamber by a clip-on spring jacket. This allows an easy exchange of the cultures to be investigated. Modifying the thickness of the gasket and/or the width of its gap, the channel geometry may be altered with the aim to use a whole range of well-defined shear stress. The flow chamber was designed to allow fluorescence microscopic observation of a perifused cell layer at high resolution.
由垫圈，其顶部 - - 流动通道的底部由主体，所述侧壁形成由盖玻片。盖玻片可以预先涂覆有在多孔板的细胞单层，并采用一个夹式弹簧护套被固定在流室中。这允许容易地交换培养物进行调查。修改所述垫圈和/或其间隙的宽度的厚度，信道的几何形状可与目的改变使用良好定义的剪切应力的整个范围。流室被设计为允许荧光显微镜观察perifused一个细胞层在高分辨率的。
